山羊副流感病毒3型感染MDBK细胞的miRNA表达谱变化分析

doi:10.11843/j.issn.0366-6964.2017.05.014

摘要/Abstract

摘要：

本研究旨在分析山羊副流感病毒3型（CPIV3）感染MDBK细胞和正常MDBK细胞差异表达的miRNA，以探索miRNA在CPIV3感染过程中的作用及其调控机制。将CPIV3感染MDBK细胞，利用高通量测序技术检测分析MDBK细胞中miRNA表达谱，并筛选差异表达的miRNA。对靶基因进行GO注释和KEGG富集。结果显示，有37个miRNA显著差异表达（P＜0.001），其中18个miRNA在病毒感染中上调，19个miRNA在病毒感染中下调。经RT-qPCR方法验证5个差异表达的候选miRNA，结果与高通量测序分析有很好的一致性。进一步GO功能注释表明：差异表达的miRNA靶基因生物学功能相对集中在免疫调节、细胞生物调控及细胞新陈代谢等生物过程。这些靶基因参与多种细胞的信号通路，包括细胞黏附分子、B细胞受体信号通路、MAPK信号通路、代谢通路、黏合斑、钙离子信号通路和趋化因子信号通路等。本研究为进一步探讨CPIV3致病机制奠定了基础。

Abstract:

To explore the function of the cellular miRNAs in MDBK cells infected with caprine parainfluenza virus type 3 (CPIV3), we compared the miRNA expression profiles in normal MDBK cells and MDBK cells infected with 10 MOI CPIV3 for 24 hours using high-throughput sequencing. GO analysis of the host target genes and KEGG enrichment were performed. The results indicated that total 37 miRNAs were obviously differently expressed in the infected MDBK cells including 18 up-regulated miRNAs and 19 down-regulated miRNAs. The five of these miRNAs were verified by RT-qPCR, the results were well matched with those of sequencing data. GO analysis of the host target genes showed that these responded miRNAs were involved in immunoregulation, biological regulation and metabolism. KEGG enrichment analysis suggested that these genes functioned in various cellular signaling pathways, including B cell receptor signaling pathway, MAPK signaling pathway, metabolic pathways, focal adhesion, cell adhesion molecules, calcium signaling pathway. Our experiment provided the data to further explore the molecular mechanism of differential expression of cellular miRNA involved in CPIV3 pathogenesis.

中图分类号:

S852.659.5

李基棕, 李文良, 毛立, 郝飞, 杨蕾蕾, 张纹纹, 江杰元. 山羊副流感病毒3型感染MDBK细胞的miRNA表达谱变化分析[J]. 畜牧兽医学报, 2017, 48(5): 896-906.

LI Ji-zong, LI Wen-liang, MAO Li, HAO Fei, YANG Lei-lei, ZHANG Wen-wen, JIANG Jie-yuan. Identification and Analysis of the miRNA Expression Profiles in MDBK Cells in Response to the Infection of Caprine Parainfluenza Virus Type 3 (CPIV3)[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(5): 896-906.

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https://www.xmsyxb.com/CN/Y2017/V48/I5/896

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